Acute oral toxicity of essential oils of cymbopogoncitratusstapf. In mice.
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The purpose of this study was to assess the acute oral toxicity profile of the essential oils of lemon grass that is used as a natural food and medicinal flavor. The oil was obtained by steam distillation of 4.6 kg of fresh lemon grass leaves using a Clevenger apparatus, dried using anhydrous sodium sulphate and its net weight taken and this was found to be 14.2 g (0.31% as yield). A stock solution of 200 g/ml was made by dissolving 4 ml of the essential oil in 20 ml of DMSO. A total of 36 (18 male and 18 Female) health mice were selected for the toxicity studies. Five different doses (4000 mg, 2000 mg, 1500 mg, 1000 mg, and 500 mg)/kg BW were administered to the grouped animals randomly in the preliminary phase of the study. Results from the preliminary phase were used to determine the dosages used in acute toxicity (500 mg, 750 mg, 1000 mg, and 1500 mg) and finally, 5 ml of the vehicle DMSO were administered to the control group. The animals were observed continuously for first 4 hours for behavioural changes and signs of toxicity and mortality within 36 hours. The values obtained were plotted against the corresponding log dose to determine the LD50. Hyper-urination, standing hair, rattling sound, hard breathing, dizziness and defecation of loose or greasy stools were some of the observable effects of the essential oil after administration. Total mortality was observed in 2,000 mg/kg BW dosage level and there was no mortality at 500 mg/kg B/w just like in control group.
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